The aquarium occupies the ground floor of Villa Piranesi – a former customs building converted into a museum complex. Upon entering, visitors walk over a glass floor revealing the archaeological remains of an 11th-century Church of St. Nicholas, the patron saint of sailors. The juxtaposition of ancient ruins underfoot and living marine world around creates an unusual atmosphere.
The 26 tanks range from 250 to 11,000 litres. The largest tank is set into the floor and holds the bigger fish species. A separate 6,500-litre tank recreates a rocky reef. Residents include sea bass, gilt-head bream, mullet, moray eels, seahorses, octopuses, dogfish sharks, starfish, sea urchins, and jellyfish. Labels beside each tank are in Slovenian, English, and Italian.
On Wednesdays and Sundays, visitors can watch feeding sessions and participate in food preparation (advance booking required). Since 2022, the aquarium has been part of the University of Primorska's Science Centre and runs sea turtle rehabilitation programmes.